FIG 4.

Transposon insertion sequence-based analyses of ibaG genetic interactions. (A) Volcano plots depicting the relative abundance of read counts mapped to individual genes in transposon libraries made in the ΔibaG mutant versus WT. For each gene, the log₂ mean fold change (x axis) and associated P value (y axis) are shown. Genes shown in color are considered significantly underrepresented compared to the WT (mean fold change of >2 and P value of <0.05), and the colors correspond to the functional classification represented in panel B. A comprehensive list of the genes over- or underrepresented in the ΔibaG library with a mean fold change of >2 and a P value of <0.05 is shown in Table S1 in the supplemental material. (B) Functional classification of the genes classified as underrepresented in the ΔibaG background. The numbers represent the percentage of genes (of 38 total) in each category. (C) Underrepresented genes in the ΔibaG insertion library that are involved in cell envelope integrity and/or LPS and peptidoglycan synthesis.