FIG 5.

IbaG interacts with iron-sulfur cluster proteins. (A) Coomassie blue-stained gel of proteins recovered after TAP purification from cell extracts of V. cholerae producing TAP-TAG only (lane 1) or IbaG-TAP-TAG (lane 2). Bands of interest were analyzed by mass spectrometry. (B) IbaG-interacting proteins identified by mass spectrometry that are iron-sulfur-containing proteins or facilitate biogenesis of iron-sulfur proteins (a complete list of interacting proteins is presented in Table S2). (C) Bacterial adenylate cyclase two-hybrid analysis of IbaG and IspG interactions. Colonies of cya-negative strains producing T25 and T18 fusions of the respective proteins on LB medium supplemented with X-Gal and IPTG are shown. (D) Lipid II quantification in WT and ΔibaG strains grown in M9 medium to exponential phase. *,  P value of <0.01 by t test.