FIG 7.

Comparison of Ccr-mCherry (RFP) to Icl-Cerulean (CFP) fluorescence in single cells over time. Succinate-grown cells of the strain P. denitrificans ccr::ccr-mCherry icl::icl-Cerulean were immobilized in a microfluidic system with a continuous flow of acetate minimal medium. Cell growth and fluorescence intensities were tracked by time-lapse fluorescence microscopy, with observation of the same field of view for 46 h. The fluorescence intensities of individual cells, determined via automated image analysis (38), are plotted as the logarithmic ratio of the RFP and CFP intensities. Gray points represent the values of individual cells. The red lines mark the medians of the sampled populations.