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. 2019 Jul 9;10(4):e00805-19. doi: 10.1128/mBio.00805-19

TABLE 1.

Genes coding for enzymes of the EMCP and the GC in Rhodobacter sphaeroides 2.4.1 and Escherichia coli K-12, respectively, and their homologues identified in P. denitrificans Pd1222a

Pathway
or cycle
Enzyme Gene
name
Gene ID in:
R. sphaeroides
2.4.1
E. coli K-12 P. denitrificans
Pd1222
EMCP β-Kethothiolase phaA RSP_0745 Pden_2026
Acetoacetyl-CoA reductase phaB RSP_0747 Pden_2027
Crotonyl-CoA carboxylase/reductase ccr RSP_0960 Pden_3873
Ethylmalonyl-CoA/methylmalonyl-CoA epimerase epi RSP_0812 Pden_2178
Ethylmalonyl-CoA mutase ecm RSP_0961 Pden_3875
(2S)-Methylsuccinyl–CoA dehydrogenase mcd RSP_1679 Pden_2840
Mesaconyl-CoA hydratase mch RSP_0973 Pden_0566
β-Methylmalyl–CoA/l-malyl–CoA lyase mcl-1 RSP_1771 Pden_0799
(S)-Malyl–CoA thioesterase mcl-2 RSP_0970 Pden_0563
Propionyl-CoA carboxylase pccAB RSP_2191/2189 Pden_3684/3688
Ethylmalonyl-CoA/methylmalonyl-CoA epimerase epi RSP_0812 Pden_2178
(2R)-Methylmalonyl–CoA mutase mcm RSP_2912 Pden_3681
GC Isocitrate lyase aceA C5Y66_09370 Pden_1363
Malate synthase aceB C5Y66_09365 Pden_1364
a

Key enzymes of the two pathways are in bold. Missing enzyme homologues are indicated by dashes.