FIG 1.

(A) Serial 0.3-μm Z stack slices through a stumpy-form trypanosome cell stained to localize the differentiation regulator TbPIP39 (green) or glycosomal TIM (red). The cell nucleus and kinetoplast are shown in blue. The TbPIP39 is located close to, but slightly anterior of, the kinetoplast and is not colocated with the glycosomal marker. DAPI, 4,6′-diamidino-2-phenylindole. Bar = 5 μm. (B) Pearson coefficient of colocalization between TbPIP39 and glycosomal TIM or between aldolase and glycosomal TIM in bloodstream slender and stumpy forms or in procyclic forms (PCF). Colocalization values were calculated using Volocity software based on captured confocal images. The threshold was set according to the background of images.