FIG 5.

Sirtinol inhibits postentry steps prior to release but not established infection. (A) U2OS cells were treated with vehicle (DMSO), ammonium chloride (NH₄Cl), or sirtinol at the indicated time relative to WNV-Kunjin (MOI of 0.5, 24 h). At 2.5 hpi, NH₄Cl was added to all wells to block viral spread. Total cellular RNA was collected and analyzed by RT-qPCR. Data represent means ± SEM. n = 3; ****, P < 0.0001 (Student's t test compared to control). (B) 293T cells were infected with WNV-Kunjin (MOI of 0.5, 24 h), or 293T cells harboring WNV-Kunjin replicons were treated with vehicle (DMSO) or sirtinol (200 μM) for 24 h, and total cellular RNA was analyzed by RT-qPCR. Data represent means ± SEM. n = 3; ***, P < 0.001 (Student's t test).