FIG 6.

Sirtinol treatment arrests dsRNA replication. (A and B) U2OS cells were left uninfected or infected with WNV-Kunjin (MOI of 1), and vehicle (DMSO) or sirtinol (200 μl) was added at 2 hpi. Following fixation at the indicated time point postinfection, cells were stained for dsRNA replication intermediates (anti-J2, green) and total nuclei (DAPI [4′,6-diamidino-2-phenylindole], blue). (B) Images representative of results of three independent experiments; representative higher-magnification images from DMSO controls are shown. (C) Quantification of granules shown in panel A. Data represent means ± SEM. n = 3; *, P < 0.05; **, P < 0.01 (Student's t test).