TABLE 1.
SPR analysis of gonococcal interactions with selected glycansa
| Glycan class | Glycan index | Glycan name | Glycan structure | KD (μM) |
|---|---|---|---|---|
| Terminal Gal | 1G | Lacto-N-tetraose (LNT) | Galβ1-3GlcNAcβ1-3Galβ1-4Glc | 0.67 ± 0.12 |
| 1H | Lacto-N-neotetraose (LNnT) | Galβ1-4GlcNAcβ1-3Galβ1-4Glc | 0.51 ± 0.08 | |
| 1N | α1-3 Galactobiose | Galα1-3Gal | 0.50 ± 0.12 | |
| Terminal GlcNAc | 4D | Hexaacetyl chitohexaose | (GlcNAcβ1-4GlcNAc)n = 3 | 0.61 ± 0.17 |
| Mannosyl | 5C | α1-2 Mannobiose | Manα1-2Man | 0.14 ± 0.06 |
| 5D | α1-3 Mannobiose | Manα1-3Man | 0.59 ± 0.28 | |
| 5H | α1-3, α1-3, α1-6 Mannopentaose | Manα1-6(Manα1-3)Manα1-6(Manα1-3)Man | 0.58 ± 0.18 | |
| Fucosyl | 7K | Blood group A | GalNAcα1-3(Fucα1-2)Gal | 3.55 ± 0.47 |
| 7M | Blood group B | Galβ1-3(Fucα1-2)Gal | 0.54 ± 0.06 | |
| 7F | Blood group O | (Fucα1-2)Gal | 0.56 ± 0.07 | |
| 7I | Lewis X | Galβ1-4(Fucα1-3)GlcNAc | 2.12 ± 0.82 | |
| 7N | Lewis Y | Fucα1-2Galβ1-4(Fucα1-3)GlcNAc | 0.28 ± 0.16 | |
| Sialylated | 10B | Sialyl-Lewis X | Neu5Acα2-3Galβ1-4(Fucα1-3)GlcNAc | 0.22 ± 0.05 |
| 11A | α2-3 Sialyllactose | Neu5Acα2-3Galβ1-4Glc | 0.77 ± 0.1 | |
| 11B | α2-6 Sialyllactose | Neu5Acα2-6Galβ1-4Glc | 0.29 ± 0.08 | |
| Terminal GalNAc | 1L | Tn antigenb | GalNAcɑ-O-Ser | N |
| Glycosaminoglycans | 13J | Heparin | (GlcA/IdoAα/β1-4GlcNAcɑ1-4)n = 200 | 1.83 ± 0.8 |
a
The affinity (dissociation constant [KD]) of the interaction between whole-cell N. gonorrhoeae and a glycan is shown.
b
Tn antigen (1L) was used as a negative control as it did not bind N. gonorrhoeae on the glycan array. N, no concentration-dependent interaction detected. Sensorgrams of all interactions are shown in Fig. S1 in the supplemental material.