TABLE 2.
Band | Accession no. | Geneb | Name | Mol wt (kDa) |
---|---|---|---|---|
1 | Q5F5H1 | NGO1955 | Uncharacterized protein | 147.2 |
2 | Q5F6Q4 | NGO1495 | Transferrin-binding protein A | 101.9 |
Q5F845 | NGO0952 | TonB-dependent receptor protein | 104.1 | |
3 | Q5F5W8 | NGO1801 | Outer membrane protein assembly factor BamA | 87.9 |
Q5F651 | NGO1715 | LPS-assembly protein LptD | 87.5 | |
Q5F7H3 | NGO1205 | TonB-dependent receptor protein | 84.9 | |
4c | Q5F969 | NGO0530 | Putative acyl coenzyme A ligase | 57.4 |
Q5F4W6 | NGO2178 | Membrane protein insertase YidC | 60.5 | |
5 | Q5F726 | NGO1363 | Multidrug efflux pump channel protein | 50.4 |
6 | Q5F693 | NGO1669 | Pilus assembly protein | 45.3 |
7c | Q5F5V7 | NGO1812 | Major outer membrane protein P.IB (PorB) | 37.2 |
Q5F8I5 | NGO0788 | Genome-derived Neisseria antigen 1220 | 34.5 | |
8 | Q5F6I1 | NGO1577 | Outer membrane protein PIII (Rmp) | 25.5 |
9c | Q5F9W0 | NGO0277 | Outer membrane protein assembly factor BamD | 30.8 |
10 | A0A0H4IV81 | NGO1073a | Opacity protein | 25.8 |
A0A0H4IV74 | NGO1040a | Opacity protein | 25.6 | |
Q5F6N6 | NGO1513 | Opacity protein OpaD | 26.6 |
Gonococcal mannose-binding proteins from gel-excised bands as identified by liquid chromatography-mass spectrometry (LC-MS) analysis. The top protein candidates from each band are shown.
Gene locus name from the N. gonorrhoeae strain FA1090 genome (GenBank accession no. NC_002946.2).
These bands were also visualized on the gel of the negative-control sample but at reduced levels.