Figure 16.

(a) The atmospheric radiofrequency (RF) atmospheric pressure He glow discharge used by Wang et al. [277], (b) schematic presentation of their mutation breeding protocol, (c) variation of the lethality rate with plasma exposure time and (d) images of the colonies before and after mutation; (left) a cultivated plate of the strains from plasma-treated spores, (W) a colony from the untreated wild strain, (Gm-1 (where m = 1–8)) the selected mutants from plasma-treated spores. Reprinted with permission from [277].