Fig. 6.

MeCP2-G428S is a loss-of-function mutant in NPC differentiation and miR-197 could reverse the defects caused by MeCP2-G428S in NPCs. a Primary mouse NPCs were infected with sh-MeCP2 lentivirus to knock-down endogenous mouse MeCP2. Sixteen hours later, these cells were infected again with control vector, WT MeCP2, or MeCP2-G428 virus (N = 4). Seventy-two hours later, cells were subjected for immunofluorescent staining. The infected cells are labeled with pseudo-colored green. The staining for MAP2, GFAP, and NESTIN are labeled with pseudo-colored red. The percentage of Nestin⁺, MAP2⁺, and GFAP⁺ cells within infected cells are shown on the right side respectively. b Primary mouse NPCs were infected with MeCP2-G428S lentivirus with either miR-control (m-cont) or miR-197 virus (N = 5). Seventy-two hours after infection, cells were subjected for immunofluorescent staining. The viruses contain EGFP and showed here as green. The miR-197/m-cont viruses infected cells are pseudo-color labeled as blue. The staining for MAP2, GFAP, and NESTIN are labeled with pseudo-colored red. The percentage of Nestin⁺, MAP2⁺, and GFAP⁺ cells within green and blue double positive cells (cyan in nucleus, white arrow head) are shown on the right side, respectively. All statistical data are presented as means ± SEM. ## p < 0.01, *** or ### p < 0.001. Scale bar is 50 μm