Fig. 7.

Regulating miR-197 reverses the effects of MeCP2 duplication and MeCP2-G428S. a Fetal Tg(MECP2) or WT FVB mice cortex were electroporated with EYFP alone or EYFP with either ADAM10 or i-197 at E14.5. All samples were collected at E18.5 for sectioning and immunostaining. Each condition was repeated in four different embryos from four pregnant female mice (N = 4). b C57BL/6 mouse were electroporated at E14.5 with either control EYFP alone or EYFP with MeCP2, MeCP2-G428S, or MeCP2-428S + miR-197 (1:1 ratio) (N = 4). Again, samples were collected at E18.5 for sectioning and immunostaining. Representative brain sections are presented on the left and the EYFP⁺ cells in each layer were counted and compared to the total EYFP⁺ cells. The statistical analyses for cells in each layer are presented on the right panel. DAPI (blue) was used for nuclear staining. Scale bar is 50 μm. All statistic data represent means ± SEM. *, # p < 0.05, **, ## p < 0.01, ***, ### p < 0.001