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. 2019 Jan 25;26(10):2115–2124. doi: 10.1038/s41418-019-0280-2

Fig. 2.

Fig. 2

Identification of the characteristic markers for SSCLCs. a Bright-field images of the induced cells at day 9, day 11, and day 13. Scale bar, 50 μm. b Gene expression dynamics of Plzf, Gfrα1, CD90, Dazl, and c-Kit during the SSCLC induction at day 9, day 11, and day 13. c Real-time PCR analyses of Plzf, Gfrα1, CD90, Mage4, Pgp9.5, Dazl, c-Kit, and Nanos3 of ESCs, day 11-induced cells, and SSCs from postnatal day 7 mice. d Immunofluorescence analysis of PLZF, GFRα1, VASA, and c-Kit of the day 11-induced cells, counterstained with Hoechst 33342. Images are representative isolated clusters positive for the indicated SSC-specific markers. Scale bar, 50 μm. e Flow cytometric analysis of VASA and PLZF expression of the day 11-induced cells. Data are represented as mean ± SEM from more than three independent experiments, *p < 0.05, **p < 0.01, ***p < 0.001.