Fig. 4.

Chronic stress evokes dysregulation of RBPs and their insoluble accumulation in P301L-Tau Tg mice. a, b Chronic stress triggered an increase in the protein levels of several RBPs and SG markers in soluble fraction [TIA-1 (p = 0.013), TLS/FUS (p = 0.017), DDX5 (p < 0.001) and EWRS1 (p = 0.005)] (a), as well in insoluble fraction of P301L-Tau mice [DDX5 (p = 0.02) and PABP (p = 0.011)] (b). c, d Chronic stress causes the cytoplasmic accumulation of the SG marker TIA-1 in hippocampus (c) and the accumulation of PABP, a SG marker (d), increasing their co-localization with p-Tau (PHF1). e Moreover, chronic stress leads to an increase co-localization of TIA-1 and PABP in perinuclear region of hippocampal neurons, indicating the presence of TIA-1 positive stress granules. f Immunofluroresecent analysis of PABP and DDX with p-Tau in human AD brain. All numeric data are represented as mean ± SEM, ^*p < 0.05; ^**p < 0.01; ^***p < 0.001