Fig. 5.

Overexpression of MORC2 attenuates C/EBPα-medicated inhibition of cell proliferation. a, b The stable expressing Flag-MORC2 of SGC-7901 cells and MCF-7 cells were transiently transfected with His-C/EBPα, and analyzed for cell growth for every 12hrs  by cell counts assay. Values are the means ± SD from three individual experiments. c The stable expressing Flag-MORC2 of SGC-7901 cells were transfected with wild type and K161R of His-C/EBPα and performed by colony formation assays. Representative results are shown. d Cell cycle analysis indicate that MORC2-C/EBPα-WT not MORC2-C/EBPα-K161R promotes cells transition from G1 to S compared to C/EBPα-WT cells and control vector cells. e, g MORC2-C/EBPα-WT and MORC2-C/EBPα-K161R cells were injected into right scapular region of the nude mice (n = 6 per group). The inoculated mice were terminated in 3 weeks. Each tumor lump was removed from the body. e Photographs of tumor weight were quantified. f Tumor volumes were measured and data are mean ± SEM. g Immunohistochemistry staining of anti-MORC2 and anti-C/EBPa were indicated in nude mice tumor tissues sections, Original magnification, ×400. Degree of intratumoral proliferation was determined by PCNA staining