Fig. 1.

Vinca alkaloids enhance IFN-induced cell death. a–c HT29 cells were treated with 10 ng/ml IFNα, IFNβ, or IFNγ and/or 100 nM VCR (a), VBL (b), or VNR (c) for 72 h. Cell death was determined by analysis of PI-stained nuclei. Mean and SD of three independent experiments performed in triplicate are shown; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. d MEFs and Capan-2 cells were treated with 10 ng/ml IFNβ (Capan-2) or 4.5 ng/ml murine IFNβ (MEFs) and/or 100 nM VNR for 72 h (Capan-2) or 48 h (MEFs). Cell death was determined by analysis of PI-stained nuclei. Mean and SD of three independent experiments performed in triplicate are shown; **P < 0.01, ***P < 0.001. e HT29 cells and MEFs were treated with 10 ng/ml IFNβ (HT29) or 4.5 ng/ml murine IFNβ (MEFs) and/or 100 nM VNR for the indicated time points. Cell death was determined by analysis of PI-stained nuclei. Mean and SD of three independent experiments performed in triplicate are shown; **P < 0.01, ***P < 0.001