Fig. 2.

Dclk1 contributes to microbiota-dependent chronic inflammation-induced epithelial repair responses in vivo and ex vivo. a IF staining on colon tissue. b Mean number of Ki67⁺ cells in distal colon crypts. c qPCR for Wnt-regulated genes in colonic crypts. Error bars, S.E.M. d Micrograph of a representative spheroid in a 3D matrigel system. Graph on right: spheroid diameter quantification; each point represents a single spheroid. e IF staining on FFPE colonoids. White arrows, colonoid buds; Yellow arrows, cells lining colonoid lumen. f Enumeration of PCNA⁺ nuclei of colon organoids. For (a–c), results are representative of 7 mice/group, ~1 month of age. For (e, f), results are representative of 3 independent experiments, with organoids from each group cultured in duplicate. For (b–d), *P < 0.05 vs. all other groups. For (f): ^†P < 0.05 vs. all other vehicle-treated groups; *P < 0.05 vs. corresponding genotype in CPCM group