Fig. 1.
TMEM268 knockout inhibits cell growth and reduces tumorigenicity. a Western blot analysis of TMEM268 expression in control cells (TMEM268 WT) and Cas9-TMEM268/BGC823 cells. b Control and Cas9-TMEM268/BGC823 cells were seeded in six-well plates (1×105 cells/well). Seventy-two hours later, representative images were obtained by optical microscopy. c Control and Cas9-TMEM268/BGC823 cells were seeded in 96-well plates (3×103 cells/well; five replicates), serum-starved for 18 h and then pulsed with 10% FCS for different lengths of time. Cell viability was detected by MTS assay. Data represent the mean ± SD of three independent experiments. d Representative images of colony formation by indicated cells. e Number of clones counted in three independent experiments. Data are expressed as mean ± SD. *P < 0.05, ***P < 0.001. f The Control or Cas9-TMEM268/BGC823 cells were injected subcutaneously in BALB/c nude mice. Development of tumors (mean volume ± SD) was monitored using calipers. g, h Excised xenograft tumors were imaged and weighed on day 19. *P < 0.05, **P < 0.01, ***P < 0.001