Fig. 2.

TMEM268 knockout causes S-phase cell cycle arrest. a Control and CAS9-TMEM268/BGC823 cells were serum-starved for 24 h and then pulsed with 10% FCS for different amounts of time. Cell cycle distribution was assayed by flow cytometry. b Percentages of G0/G1, S, and G2/M phase cells. Each bar represents the mean ± SD of three independent experiments. c Cells were treated as described in (a), and protein levels detected by western blotting. d Quantification of the levels of the indicated proteins relative to GAPDH in cells treated as described in (a). Average value for control cells (24 h) was normalized to 1. Data shown represent the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001