Fig. 3.

TMEM268 knockout decreases cell adhesion. a Control and Cas9-TMEM268/BGC823 cells were allowed to attach to 96-well plates precoated with fibronectin or laminin. The unadhered cells were removed at 30 min, and the number of adhered cells was determined using an MTS assay (*P<0.05, **P<0.01). b−f Control and Cas9-TMEM268/BGC823 cells were plated on glass slides for 24 h, incubated with the indicated FITC-conjugated antibodies. Nuclei were stained with Hoechst 33342. Representative images obtained from confocal microscopy are shown. Scale bars, 10 μm