Fig. 1.

Pdgfra was expressed in the peri-cloaca and genital tubercle mesenchyme of mouse embryos from E10.5 to E14.5. Immuno-histochemistry for Pdgfra was performed on transverse section of E10.5 and mid-sagittal sections of E11.5–E14.5 mouse embryos. Immuno-reactivity for Pdgfra (brown) was localized at the mesenchyme surrounding the cloaca of the budding genital tubercle at E10.5. From E11.5 to E13.5, Pdgfra expression was detected at the genital tubercle, urorectal septum and the hindgut mesenchyme. At E14.5, a more restricted expression of Pdgfra was localized at the mesenchyme adjacent to the urethra and the ureter, at the urorectal septum and at the urethral plate mesenchyme. cl cloaca, hlb hindlimb bud, nt neural tube gt genital tubercle, ugs urogenital sinus, urs urorectal septum, hg hindgut, cm cloaca membrane, t tail, ur urethra, up urethral plate, ud urethral duct, ao anal opening, u ureter, r rectum