Fig. 6.

CALCOCO2 is cleaved after Q139 by viral proteinase 3C. a HeLa cells were transfected with 3C^wt together with GFP-CALCOCO2^WT or GFP-CALCOCO2^Q139L as indicated. After 24 h, cell lysates were harvested and subjected to western blot analysis using an anti-GFP antibody. b In vitro cleavage assay was conducted by incubation of lysates of HeLa cells transfected with GFP-CALCOCO2^WT or GFP-CALCOCO2^Q139L with recombinant 3C^wt, 3C^mut, or vehicle (−) as above in e. c Schematic illustration of the structural domains, the identified cleavage site, the antibody recognition regions, and the resulting cleavage products of CALCOCO2. SKICH skeletal muscle and kidney-enriched inositol phosphatase (SKIP) carboxyl homology, CLIR LC3C-interacting region, LIR LC3-interacting region, CC coiled-coil domain, UBZ ubiquitin-binding zinc finger region. Arrows denote the cleavage fragments. NS non-specific bands. Results in this figure represent data from two independent experiments