Fig. 3.

Dcaf13 knockout in oocyte results in follicular development defect. a and b Immunohistochemistry staining of forkhead box O1 (FOXO1, a marker of granulosa cells in growing follicles (a)) and mouse VASA homolog (MVH, a germ cell marker (b)) in the ovaries of 5-month-old mice with indicated genotypes. Scale bars, 500 μm. c In vitro developmental analysis of follicles derived from Dcaf13^fl/fl or Dcaf13^fl/fl;Gdf9-Cre mice. Days in culture were indicated. The asterisk indicates follicular antrum. Scale bars were shown in each picture. For each genotype, at least 40 follicles were cultured. d Quantification of developmental rates for in vitro cultured follicles in c. PF primary follicles, Bi secondary follicles with two layers of granulosa cells, MuF secondary follicles with multiple layers of granulosa cells, AF antral follicles, d degenerated follicles