Fig. 8.

Summary of DCAF13 function in rRNA processing and oocyte and follicle development. a During follicle development, growing oocytes undergo a translationally active growing phase in which necessary protein are synthesized. DCAF13 deletion in oocytes compromised nucleolus NSN–SN configuration transition and caused global downregulation of protein accumulation, which cause follicular development arrest in early secondary follicles. b In the nucleolus, the 47S ribosomal precursor RNA (pre-rRNA) is cleaved to form the mature 28S, 18S, and 5.8S rRNAs. During this maturation process, the pre-rRNA and its processing intermediates undergo numerous post-transcriptional modifications, which are guided and catalyzed by small nucleolar RNAs (snoRNPs) and their interacting proteins including NOP56 and fibrillarin. DCAF13 has a strong interaction with NOP56 and fibrillarin, which are associated with box C/D U3 snoRNA, to participate in the 18S rRNA processing. DCAF13 ensures enough mature 18S rRNA in the cytoplasm to assemble 40S subunit of ribosomes and regulates protein translation