Fig. 5.

Accurate prediction of sensitisation to trametinib by combined knock down of c-JUN and FAK. a Vectors indicate the movement for WM1205-LU cells within the 3D PC space, as predicted by co-depleting c-JUN and FAK. WM1205-LU cells move towards regions populated by responsive cell lines upon co-depleting c-JUN and FAK. b Experimental validation of prediction. c-JUN or FAK were co-depleted by siRNA transfection (48 h), and cells were treated with trametinib and Q-VD-OPh for 48 h. Cell death was determined using PI/Annexin V-GFP staining (means + S.E.M. from n = 3 independent experiments). Statistical analysis was performed using Student's t-test