Fig. 6.

SPOP plays opposite roles in PCa and CCRC tumorigenesis. a–c Xenograft experiments were performed in nude mice with DU145 or 769-P cells stable expressing SPOP. The resulted tumor tissues were pictured (a). Tumor volumes (b) and weights (c) of DU145 were shown as mean ± SEM, n = 9. ***means p < 0.005. d DU145, PC3, 769-P, and 786-O cells were transfected with control or two independent SPOP-specific siRNAs. Seventy-two hours after transfection, cells were harvested for WB. e DU145 or 769-P cells infected with lentiviral empty vector or SPOP was assayed with WB. f Venn-diagram shows the overlapped genes of DEGs between TCGA human cancer samples (PCa+CRCC) and DEGs of SPOP RNAi (DU145+769-P). g Heat map of the overlapped genes in f. Cancer-related genes were labeled with red color