Fig. 2.

Bladder cancer cells with ARID1A and/or SWI-SNF mutations are sensitive to EZH2 inhibition. a 1 µM EPZ011989 alone or in combination with 0.25 µg/ml cisplatin reduced the number of viable cells compared to vehicle. The Y-axis represents the percentage of cells normalized to the vehicle, and the X-axis represents the cell lines used in the study. b EPZ011989 alone or in combination with cisplatin increased the number of cells in the G2/M phase of the cell cycle. The Y-axis represents the percentage of propidium iodide positive cells, and the X-axis represents the different phases of the cell cycle. c The image is a representative of clonogenic assay performed on all three cell lines. EPZ011989 alone or in combination with cisplatin significantly reduced the number of clones. The Y-axis represents the surviving fraction of clones normalized to the vehicle. Error bars represent standard error of means from technical duplicates and biological triplicates. Students t-test was used to analyze significance between the single agent and combination treatments. *p < 0.05, **p < 0.01, ***p < 0.001, triangle indicates significant difference compared to vehicle, hash indicates a significant difference between the EPZ011989 and cisplatin alone treatments