Fig. 5.

Combined loss of caspases-1, -11 and -12 does not protect mice against ER stress-induced renal damage. a Immunohistochemical staining of kidneys from tunicamycin-treated (1 mg/kg body weight, harvested after 72 h) and -untreated mice to detect TUNEL⁺ (dead) cells of WT mice are shown in the left panels, and for caspase-1/11/12 triple knockout mice in the right right panels. One representative image shown per genotype and treatment. n ≥ 3. Sections: cortex (1), outer medulla (2) and inner medulla (3). Scale bar represents 1 mm, total magnification of kidney ×20. b Bar graphs showing quantification of results from a n ≥ 3 mice per genotype, two kidney sections per mouse were scored computationally