Fig. 3.
BH3-only proteins have different binding preferences in various solid tumor cell lines despite similar sensitivities to BH3 mimetics. a Solid tumor cell lines were treated with a concentration range of S63845 and a constant concentration of 100 nM of A-1331852 for 4 h and assessed for PS externalization. Immunoprecipitation of MCL-1 and BCL-XL was carried out in H1299 b, SUIT-2 c, and HCT116 d cells exposed to S63845 (100 nM) or A-1331852 (100 nM) for 2 h, and the eluted complexes were immunoblotted for the indicated proteins. * in the blots denotes non-specific bands. Error bars = Mean ± SEM (standard error of the mean); *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001