Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Nov 21;26(9):1582–1599. doi: 10.1038/s41418-018-0226-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — Identification of the interaction between Vps26a and Nox4. a Serial sagittal sections of WT embryos were immunostained for Vps26a, Nox4, and Tubb3 and counterstained lightly with hematoxylin at E10.5. da, dorsal aorta; l, lung; h, heart; nc, notochord; nl, neural lumen; nt, neural tube; som, somite. b Vps26a immunoprecipitation followed by anti-Vps26a and -Nox4 immunoblots using lysates obtained from WT (+/+) and Vps26a^-/- (-/-) ESCs during neural differentiation for 0 or 6 days. IgG was used as an immunoprecipitation control. c GST-tagged Vps26a was subjected to a pull-down assay with the lysates of HEK293 cells transfected with HA-Nox4C (C-terminal region, 249–574 aa)-expressing plasmid. Immunoblot analysis with anti-HA antibody is shown at the top. Equal loading of the GST proteins assessed by GST antibody is shown at the bottom. GST was used as a negative control. d Cell lysates from HEK293 cells transfected with HA-Nox4C were mixed with purified His-tagged Vps26a. Samples were immunoblotted with the HA tag antibody. Levels of input protein are shown by immunoblotting with the HA- and Myc-tag antibodies. e Expression constructs for GST-tagged serial deletion mutants of Vps26a and HA-Nox4C were co-expressed in HEK293 cells. Immunoblot analysis with anti-HA antibody is shown at the top. Equal loading of the GST proteins assessed by GST antibody is shown at the bottom. GST was used as a negative control