Fig. 3.

miR-142-3p is repressed by hypermethylation mediated by EZH2-recruited DNMT1. a Schematic location of the core DNA motif (red) of the polycomb response element (PRE). b, c PCR (b) and CHIP-qPCR (c) assay for anti-EZH2 and H3K27me3 in NPC cells. d Western blot analysis of EZH2, H3K27me3, and Histone3 in NPC cells with EZH2 overexpression or downregulation or control. e CHIP-qPCR assays for NPC cells after EZH2 modification. f, g Real-time PCR (f) and methylation level (g) assays for NPC cells after DNMTs downregulation. h Physical interactions between EZH2 and DNMT1 were examined via a Co-IP assay in NPC cells with EZH2 overexpression or downregulation. i PCR assay for DNMT1 occupancy in NPC cells. j CHIP-qPCR assays for anti-DNMT1 in NPC cells after EZH2 downregulation. Mean (n = 3) ± s.d. Student’s t test, *P < 0.05, **P < 0.01