Fig. 7.

Administration of miR-142-3p suppresses NPC cell aggression in mouse model. a SUNE-1 cells (2×10⁵ cells in 30 µl PBS) were inoculated into mice foot-pads. After 2 weeks’ growth, intratumoral injection of agomir to overexpressing 142-3p or agomir NC control were injected into foot-pad tumors twice a week for 2 weeks. b Representative images of microscopy foot-pad tumors. Scale bar, 100 μm. c Representative images and quantification of inguinal lymph nodes. Mean (n = 7) ± s.d. Student’s t test, **P < 0.01. d Representative images of pan-cytokeratin-positive tumor cells and metastatic ratios of inguinal lymph nodes. *P < 0.05, scale bar, 100 μm. e, f Representative images and quantification of macroscopic (e) and microscopic metastatic nodules (f) in the lungs of mice after systemic administration of agomir-miR-142-3p or agomir control. Mean (n = 5) ± s.d. Student’s t test, **P < 0.01. Scale bar, 100 μm