Fig. 4.

Western blot analysis of autophagy-related proteins expression in EpoCD34/HSPCs. a PI3K/AKT/mTOR/ULK1/Atg13 pathway of untreated or treated cells as indicated or transfected with si-SMPD3 at 96 h. b Beclin-1, Atg5-12, Atg7, p62/SQSTM1, and Rubicon in untreated CD34/HSPCs at 0 h and in EpoCD34/HSPCs untreated or treated as indicated at 24 and 96 h. β-Actin expression was used in Western blot analysis as an internal control and was revealed each time on the same membrane than one or several proteins of interest