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. 2018 Oct 25;26(8):1501–1515. doi: 10.1038/s41418-018-0225-1

Table 3.

Apoptotic cell counts in the ventral spinal cords of E13 double heterozygous (Bcl-x HET:Bax HET), BKO, and BKO:Bax Null Embryos

Bcl-x HET:Bax HET BKO BKO:Bax Null
Apoptotic cells 0.0 ± 0.0 30.2 ± 8.1 0.0 ± 0.0
Total nuclei 151.5 ± 19.8 153.5 ± 8.7 145.5 ± 16.8
% Apoptotic cells 0.0 ± 0.0 19.6 ± 4.7* 0.0 ± 0.0
# of Embryos 4 4 4

Apoptotic cells were identified as immunopositive for active Caspase-3 with completely condensed nuclei. Cells were counted within a 150 µm × 150 µm box placed in the ventrolateral horn of the spinal cord. Means ± SD are indicated

*p  < 0.0001