Table 3.
Bcl-x HET:Bax HET | BKO | BKO:Bax Null | |
---|---|---|---|
Apoptotic cells | 0.0 ± 0.0 | 30.2 ± 8.1 | 0.0 ± 0.0 |
Total nuclei | 151.5 ± 19.8 | 153.5 ± 8.7 | 145.5 ± 16.8 |
% Apoptotic cells | 0.0 ± 0.0 | 19.6 ± 4.7* | 0.0 ± 0.0 |
# of Embryos | 4 | 4 | 4 |
Apoptotic cells were identified as immunopositive for active Caspase-3 with completely condensed nuclei. Cells were counted within a 150 µm × 150 µm box placed in the ventrolateral horn of the spinal cord. Means ± SD are indicated
*p < 0.0001