Fig. 8.

PGC1β-OT1 regulated differentiation of progenitor cells via its downstream effectors. PGC1β-OT1 construct or the vector was cotransfected with miR-148a-3p or control agomir into ST2, and differentiated adipocytes were stained by oil red O (a). Oil red O extracted with isopropanol was measured at OD520 (b). The mRNA levels of adipogenic factors were examined (c). qRT-PCR analysis was performed to verify the silencing of PGC1β-OT1 and overexpression of Kdm6b (d). siPGC1β-OT1 or control small-interfering RNA was cotransfected with Kdm6b expression construct or vector, and differentiated osteoblasts were subjected to alkaline phosphatase staining (e). The mRNA levels of osteogenic factors were examined (f). Data are mean ± SD, n = 3. *p < 0.05 vs. NC plus Vector. ^#p < 0.05 vs. miR-148a-3p agomir plus Vector (b, c) or siPGC1β-OT1 plus Vector (f)