Fig. 1.

CD122-Cre mediated pdk1 deletion severely compromises NK cells development. a-c Representative flow cytometry plots (a, b) and quantification (c) of hematopoietic stem cells (HSC, Lin⁻CD127⁻c-Kit⁺Sca-1⁺), common myeloid progenitors (CMP, Lin⁻CD127⁻c-Kit⁺Sca-1⁻) and common lymphoid progenitors (CLP, Lin⁻CD127⁺c-Kit⁺Sca-1⁺) (A), pre-NKp (Lin⁻CD127⁺2B4⁺CD135⁻CD112⁻) and NKp (Lin⁻CD127⁺2B4⁺CD135⁻CD112⁺) (b) in the BM of WT and PDK1^Vav1-Cre mice. Numbers near the indicated square box show the respective percentage. d, e The absolute number of T cells (d) and B cells (e) in the indicated tissues and organs from WT and PDK1^CD122-Cre mice. f, g Representative flow cytometric profiles (f) and the absolute number (g) of NK-T cells (CD3^lowNK1.1^low) in the spleens and livers of WT and PDK1^CD122-Cre mice. h, i Representative flow cytometric profiles (h) and the absolute number (i) of NK cells (CD3⁻NK1.1⁺) in the spleen, BM, LN, liver, and lungs of WT and PDK1^CD122-Cre mice. j, k Representative flow cytometric profiles (j) and the percentages (k) of NK cell subsets in the spleen and BM from the WT and PDK1^CD122-Cre mice. DN (CD27⁻CD11b⁻), CD27 SP (CD27⁺CD11b^-), DP (CD27⁺CD11b⁺) and CD11b SP (CD27^-CD11b⁺) cells. l Percentage of developmental markers on splenic NK cells (CD3⁻NK1.1⁺) in WT and PDK1^CD122-Cre mice. m Representative flow cytometry plots and quantification of BM and liver ILC1 (CD3⁻NK1.1⁺CD49a⁺CD49b⁻). The data represent one of three independent experiments, and values are expressed as the mean ± s.d