Fig. 2.
CD122-Cre mediated pdk1 deletion impairs NK cell-mediated immunosurveillance. a, b Intracellular staining of IFN-γ (a) and CD107a expression (b) on poly (i:c)-activated splenic NK cells. c Representative flow cytometry (left) and the percentage (Right) of β2m-deficient splenocytes rejection in WT and PDK1CD122-Cre mice. R1, CFSE-low splenocytes from WT mice; and R2, CFSE-high splenocytes from β2m-deficient mice. d Representative flow cytometry (left) and the percentage (right) of RMA-S cell rejection 18 h after intraperitoneal injection of the indicated mice with a mixture of NK cell-sensitive RMA-S-GFP cells together with NK cell-resistant RMA-DsRed. e For the B16 metastasis assay, the indicated mice pretreated with or without antibody against NK1.1 were injected intravenously with 2 × 105 B16 cells. 14 days later, and the lung weights and numbers of tumor nodules were counted. Each symbol represents an individual mouse. The data represent one of two independent experiments, and values are expressed as the mean ± s.d