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. 2018 Oct 12;26(8):1379–1395. doi: 10.1038/s41418-018-0211-7

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© The Authors 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — RASSF1A helps recruit autophagosomes onto acetylated microtubules through MAP1S and regulates autophagy initiation and autophagosomal degradation. a Representative immunoblots showing levels of MAP1S and LC3 associated with microtubules assembled in vitro. MT, assembled microtubules. β-Tubulin serves as the marker for assembled microtubules. b, c Representative fluorescent images showing the impact of RASSF1A (blue) on the distribution of GFP-FL MAP1S (green) and acetylated α-tubulin (red) (b) or RFP-LC3 (red) (c) in HeLa cells transiently expressing HA-tagged RASSF1A and GFP-tagged FL (b) or HA-tagged RASSF1A, GFP-tagged FL and RFP-LC3 (c). d A diagram showing the mechanism by which RASSF1A promotes autophagosomal trafficking. e, f Representative immunoblots showing the levels of p-S70S6K, LC3, and γ-H2AX in hepatocytes isolated from wild-type and RASSF1A^−/− mice (e) or HeLa cells treated with random (Mock) and RASSF1A-specific siRNAs (RA) (f) untreated (Ctrl) or treated with rapamycin (Rapa) in the absence (None) or presence of bafilomycin A1 (BAF). g Representative immunoblots showing the levels of p-Akt, p-S70S6K, LC3, and γ-H2AX in wild-type HeLa cells (+/+) or HeLa cells with RASSF1A knockout (−/−) transiently transfected with no plasmid (No), control vector (Ctrl), HA-RA or HA-RA∆ in the absence (None) or presence of bafilomycin A1 (BAF). h Representative immunoblots showing the levels of p-Akt, p-S70S6K and LC3 in wild-type HeLa cells (+/+) or HeLa cells with RASSF1A knockout (−/−) transiently transfected with no plasmid (No), control vector (GFP), GFP fused fragments in the absence (None) or presence of bafilomycin A1 (BAF)