Fig. 2.

Hypoxia downregulates TSC1. a Expression levels of components of the mTOR signaling pathway, such as mTOR, TSC1, TSC2 and RHEB, were compared in PASMCs exposed to normoxia or hypoxia by NGS-based RNA sequencing. b Expression levels of TSC1 mRNA normalized to 18S rRNA were examined by qRT-PCR in PASMCs exposed to normoxia or hypoxia for 8 h or 24 h. *p < 0.05. c PASMCs were treated with 25 ng/ml actinomycin D for 2 h prior to hypoxia exposure for 8 h or 24 h. Level of TSC1 transcripts relative to 18S rRNA was measured by qRT-PCR. *p < 0.05. d Total cell lysates from PASMCs exposed to normoxia or hypoxia for 24 h were subjected to immunoblot analysis with anti-TSC1 or anti-β-actin antibody. By densitometry, relative amounts of TSC1 protein normalized to β-actin were quantitated. *p < 0.05