Fig. 6.

miR-92b-3p-mediated inhibition of TSC1 modulates mTOR signaling. PASMCs transfected with TSC1-expressing vector containing the miR-92b-3p binding site (Control) or TSC1 expression construct carrying the TSC1 cDNA deleted in the 3′-UTR (TSC1^wo3′^UTR) were exposed to normoxia or hypoxia for 24 h. Total cell lysates were subjected to immunoblot analysis with antibody against TSC1, pS6K1, S6K1 or β-actin. By densitometry, relative amounts of TSC1 protein normalized to β-actin and phosphorylated S6K1 protein normalized to total S6K1 were quantitated. *p < 0.05