Fig. 7.

miR-92b-3p regulates mTOR signaling and VSMC proliferation. a, b Total cell lysates from PASMCs transfected with control mimic, miR-92b-3p mimic (a), or anti-miR-92b-3p (b) were subjected to immunoblot analysis with antibodies against pS6K1, S6K1 or β-actin. By densitometry, relative amounts of phosphorylated S6K1 protein normalized to total S6K1 were quantitated. *p < 0.05. c Representative microphotographs of Ki-67 immunostaining of PASMCs transfected with control mimic, miR-92b-3p or anti-miR-92b-3p, and calculation of Ki-67 index. Approximately 200 cells from at least 10 independent fields were counted for each condition, and Ki-67-positive cells are presented as a percentage of the total population. Scale bar represents 50 μm. *p < 0.05