Fig. 8.

Modulation of miR-92b-3p controls mTOR signaling-mediated VSMC proliferation induced by hypoxia. a PASMCs transfected with control mimic or anti-miR-92b-3p were exposed to normoxia or hypoxia for 24 h. Total cell lysates were subjected to immunoblot analysis with antibody against pS6K1, S6K1 or β-actin. Relative amounts of phosphorylated S6K1 protein normalized to total S6K1 were quantitated by densitometry. *p < 0.05. b Representative microphotographs of Ki-67 immunostaining. PASMCs transfected with control mimic, miR-92b-3p or anti-miR-92b-3p were exposed to normoxia or hypoxia and then subjected to immunofluorescence staining with anti-Ki-67 antibody. Approximately 200 cells from at least 10 independent fields were counted for each condition, and Ki-67-positive cells are presented as a percentage of the total population. Scale bar represents 50 μm. *p < 0.05