Fig. 7.

Effect of BICD1 regulation on skin wound healing capacity of UCB-MSC transplantation. a–e Mouse skin wound surgery with vehicle or UCB-MSC transplantation was conducted as described in Materials and methods. HP-MSC indicates mice group given hypoxia-pretreated UCB-MSCs with NT siRNA. a Skin wound size was compared with post-injection day 0. n = 12. b Tissue samples at post-injection day 10 were stained with hematoxylin and eosin. Scale bars are 200 μm (Magnification, × 40). n = 7. c Vessel distribution was quantified with ImageJ software. n = 10. d, e Tissue slides were immunostained with CD31, HNA, and α-SMA-specific antibodies. The percentages of CD31, HNA, and α-SMA-positive cells in DAPI-positive were analyzed by using Metamorph software. Scale bars are 100 μm (Magnification, × 100). n = 4. Quantitative data are presented as a mean ± S.E.M. All gross and immunofluorescence images are representative. ^$p < 0.05 vs. vehicle group, *p < 0.05 vs. MSC group with NT siRNA, ^#p < 0.05 vs. HP-MSC with NT siRNA. N.D. indicates not detected