Fig. 3.

Increased DR in MCDD-fed c-Jun^∆li mice. a The number of CD3-, F4/80-, Ly6G-, and NKp46-positive cells was assessed by immunohistochemistry (n = 4-16/genotype and timepoint). b Quantification of Ki67-positive NPLCs by immunohistochemistry (n = 4–9/genotype and timepoint). c Sox9-positive cells were assessed by immunohistochemistry (n = 5–15/genotype and timepoint). d–f Hepatic expression of Opn and of its receptor CD44 was determined by qPCR (d and e, n = 4–8/genotype and timepoint) and immunohistochemistry (f, representative stainings following 49 days of MCDD). mRNA expression is shown relative to untreated controls. Significance was tested by Mann–Whitney test. P-values are indicated if significant. Scale bar = 50 µm