Fig. 6.

xCT inhibition causes ferroptosis. a WT and Tsc2^−/− MEF cells were treated with SASP for 48 h at the indicated concentration in triplicate. Cell viability was determined by counting viable cell numbers. b Tsc2^−/− MEF cells were treated with SASP or its solvent DMSO. ROS levels were detected with DCFH-DA and flow cytometry. Ctrl: no treatment control; H₂O₂: positive control. Cell counting of Tsc2^−/− MEF cells treated with 0.15 mM SASP combined with 5 mM NAC (c) or 2 uM Ferr-1 (d) for 48 h. Cell counting of Tsc2^−/− MEF cells treated with 0.5 mM SASP combined with 2 μM Z-VAD-FMK (e) or 5 μM Nec-1 (f) for 48 h. *P < 0.05, **P < 0.01, ***P < 0.001. Data are presented as means ± SEM