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. 2018 Oct 19;26(6):1077–1088. doi: 10.1038/s41418-018-0215-3

Fig. 4.

Fig. 4

Poly(I:C) transfection-induced RIG-I, MDA-5, and caspase-11 expression in MEFs is NF-κB dependent. a Effect of NF-κB inhibitor BMS-345541 on poly(I:C) transfection-induced RIG-I, MDA-5, and caspase-11 expression at the indicated times in Abin-1+/+ and Abin-1−/− MEFs. BMS-345541, 8 μM, pretreatment for 30 min; poly(I:C), 2 μg/ml. b Effect of NF-κB inhibitor IKK-16 on poly(I:C) transfection-induced RIG-I, MDA-5, and caspase-11 expression at the indicated times. IKK-16, 5 μM, pretreatment for 30 min; poly(I:C), 2 μg/ml. c Effect of Abin-1 knockout and Abin-1/Ripk3 double knockout on RIG-I, MDA-5, p-IRF-3, and caspase-11 expression upon poly(I:C) transfection for 4 and 8 h in primary MEFs. Poly(I:C), 2 μg/ml. d Effect of TBK1 inhibitor MRT67307 on poly(I:C) transfection-induced RIG-I, MDA-5, and caspase-11 expression at the indicated times. MRT67307 (MR), 2 μM; poly(I:C), 2 μg/ml. Results shown are representative of at least three independent experiments