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. 2018 Sep 25;26(7):1235–1250. doi: 10.1038/s41418-018-0202-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — The expression level of TC2N is correlated with lung cancer progression. a The measurements of TC2N expression in lung cancer cell lines compared with the normal cell line HBE by qRT-PCR and WB. ACTIN is shown as a loading control. The data were analyzed using the formula 2^‒Δct. Data represent the mean ± SD of triplicates. b, c The protein expression of TC2N in adjacent and tumor lung samples monitored by immunohistochemistry (IHC) on a tissue array. The anti-TC2N antibody was employed for IHC. IHC scoring was performed blind, prior to association with clinical data. d IHC analysis on a tissue array of lung cancers at different histological grades. e Immunohistochemistry analysis on a tissue array of lung cancers at different clinical stages. Scale bars represent 50 μm. f Kaplan–Meier analysis of TC2N expression in 272 lung cancer patients subdivided into two groups. g Kaplan–Meier analysis of TC2N expression in 272 lung cancer patients subdivided into three groups *P < 0.05