Fig. 3.

TC2N promotes tumorigenesis of lung cancer cells in nude mice. a Examination of tumorigenesis in subcutaneously injected animals using H460 cells stably expressing the negative control or TC2N shRNA. The tumor growth curve of shRNA-expressing cells was compared with negative control cells. b The fluorescent images of xenograft tumors from nude mice subcutaneously injected with H460 stable transfectants were photographed. c Tumor weight from the negative control or TC2N shRNA groups. Each group contained seven mice. d The tumor growth curve was performed in nude mice using H1975 cells stably expressing vector control or TC2N. e Xenograft tumors from (d) were dissected and photographed. f Tumor weight from the vector control and TC2N groups. Each group contained six mice. g Knockdown of TC2N increases the number of apoptotic H460 cells in xenograft tumors. Two representative images were randomly selected from each group (H460-NC, H460-shRNA1 and H460-shRNA2). Scale bars represent 50 μm *P < 0.05; **P < 0.01;***P < 0.001