Fig. 8.

Inhibition of TC2N increases cell apoptosis with doxorubicin treatment. a H460 cells were incubated with 1 µM Dox for 0 to 24 h, as indicated. Cells were harvested, and then indicated protein levels were determined by WB. b H460 cells stably expressing the negative control or TC2N shRNA were treated with Dox (1 µM) for 24 h and then analyzed by flow cytometry for apoptosis. Results are the mean ± SEM of three independent experiments. *P < 0.05. c The protein expression determined by WB were depicted for the cells treated as the same in (b). d Schematic diagram for the potential mechanisms of TC2N-mediated promotion of tumorigenesis in lung cancer