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STRA8 was expressed in germ cells of both male and female Wt1R394W/R394W mice at E12.5 and E13.5. STRA8/DAZL double-staining experiment was performed with control (Wt1+/R394W) and Wt1R394W/R394W embryos at E11.5 (A–D), E12.5 (E–H), and E13.5 (I–L). Germ cells were labeled with DAZL (green). DAPI (blue) was used to stain the nuclei. The arrowheads point to STRA8-positive germ cells in Wt1R394W/R394W gonads. The gender of the embryos was confirmed with PCR using Sry primers.
